A quantitative stopped-flow fluorescence assay for measuring polymerase elongation rates
نویسندگان
چکیده
منابع مشابه
A quantitative fluorescence‐based steady‐state assay of DNA polymerase
Fluorescent dyes that bind DNA have been demonstrated as a useful alternative to radionucleotides for the quantification of DNA and the in vitro measurement of the activity of DNA polymerases and nucleases. However, this approach is generally used in a semi-quantitative way to determine relative rates of reaction. In this report, we demonstrate a method for the simultaneous quantification of DN...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2009
ISSN: 0003-2697
DOI: 10.1016/j.ab.2009.04.035